Journal: Cell reports

Article Title: STK25 inhibits PKA signaling by phosphorylating PRKAR1A

doi: 10.1016/j.celrep.2022.111203

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: SmartSeq Ultra Low V4 kit , Clontech/Takara , R400752.

Techniques: Recombinant, SYBR Green Assay, In Vitro, Activity Assay, Viability Assay, Sequencing, esiRNA, CRISPR, Knock-Out, Plasmid Preparation, Control, Software

Journal: bioRxiv

Article Title: MiR-137 and miR-122, two outer subventricular zone-enriched non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation

doi: 10.1101/2021.04.01.438039

Figure Lengend Snippet: ( A ) Left, schematic diagram of the experiment to distinguish L2/3 neurons (born from E15.5 on) from L4 (born at E14.5) neurons after E14.5 in utero electroporations. Middle, EdU staining at P3. Right, Quantifications of EdU+ cells in superficial (SL) and deep (DL) layers/ white matter (WM) upon scram or miR-122 overexpression. ( B ) Left, schematic diagram of miR-122 SL and DL P7 neuron isolation after microdissection, bulk RNA-sequencing and support vector machine learning approach to predict SL vs. DL neuron position. Right, Prediction of miR-122 single cells using this model. ( C ) Left, schematic diagram of L2/3 and L4 P3 and P7 neuron isolation through birthdate-locked in utero electroporation (IUE), single-cell RNA sequencing and support vector machine learning approach to predict P3 vs . P7 identity ( ie . maturation score). Right, maturation and layer identity (ID) scores of P3 and P7 E14 / E15.5 born neurons. ( D ) Passive properties and spontaneous activity recordings in L2/3 scrambled (scram) and miR-122 neurons at P21. Passive properties: Ih current, Capacitance, Input resistance, Membrane constant, Sag ratio, Spike threshold, AHP, Rheobase, Spike peak, Spike ratio, RMP, V drop , V min , V end , Spike delay. ( E ) CASP3 expression in cortex at P7. Quantification of CASP3+ in scram and miR-122 overexpressing cells. Data are represented as mean ± SEM (A, E) or mean ± SD (D). (A) Two-way ANOVA; D-E, Unpaired t-Test. Biological replicates and recorded cells are distinguished by circles in the bar plots (A and D, respectively). *p < 0.05, **p < 10 -2 , ***p < 10 -3 , ****p < 10 -4 .

Article Snippet: RNA from each sample was extracted using Total RNA Isolation System kit (Promega SV) and quality control was done using 2001 Bioanalyzer from Agilent. cDNA libraries were obtained using SMARTseq v4 kit (Clontech, # 634888) and sequenced using HiSeq 2500 sequencer.

Techniques: In Utero, Staining, Over Expression, Isolation, Laser Capture Microdissection, RNA Sequencing Assay, Plasmid Preparation, Electroporation, Activity Assay, Expressing

Journal: Cell reports

Article Title: Cell type specializations of the vocal-motor cortex in songbirds

doi: 10.1016/j.celrep.2023.113344

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The dorsal/ventral axis was defined as orthogonal to the descending occipito-mesencephalic tract (OM), and subregions dissected based on the pattern of retrogradely labeled cells in RA from previous studies., , RNA from these samples were isolated using a Qiagen RNeasy Micro Kit and cDNA libraries were generated using Takara Bio SmartSeq v4 PLUS kit.

Techniques: Virus, Plasmid Preparation, Recombinant, Labeling, Expressing, Clone Assay, In Situ, Software